LeadArt Technologies

PROTEOMICS SERVICES

Chemical Proteomics, Peptidomics, and Proteomics

Drug Target Identification and Chemical Proteomics

• LeadArt Technologies provides closed-loop services from active compounds to target identification

• Probe synthesis and target identification are customized service items of chemical proteomics

With the successful implementation of the Human Genome Project, the focus of life science research has gradually shifted to the overall study of biological functions. The research on the expression pattern and function pattern of protein, the main embodiment or executor of biological function, has become the development trend of life science.
The concept of proteome was first proposed by Australian scholars in 1995. It is derived from the heterozygosity of protein and genome, which refers to all proteins expressed by a genome, a cell tissue or an organism in a specific time and space. The research on proteomics is mainly to study the composition and activity of proteins in cells at the overall level.
The specific research contents of proteomics include: protein qualitative and quantitative analysis, protein post-translational modifications, protein structure, protein function, small molecule-protein and protein-protein interaction, etc. Quantitative methods contain label free and label(SILAC, iTRAQ, TMT, etc.).
When the latest developments in chemistry and biology merged, a new interdisciplinary research field was created, namely chemical proteomics or chemoproteomics. Chemical proteomics is a new field which is still expanding and has not been definitively defined in the academic circles. As a new technology in the post-genome era, it is the bridge and link between proteome and drug target research, as well as drug discovery. Based on the current development direction and research results of chemical proteomics, it can be considered that the research method of chemical proteomics is to use chemical small molecules that can specifically interact with target proteins to interfere with and detect the proteome, to systematically reveal the function of specific proteins and their interaction with chemical small molecules at the molecular level, so as to accurately find the binding site of small chemical molecules (including drugs), that is, the targets.
The techniques of chemical proteomics include: activity-based protein profiling (ABPP), affinity-based protein profiling (AfBPP), thermal protein profiling (TPP) , drug affinity responsive target stability (DARTS), limited proteolysis-small molecule mapping(Lip-SMAP), stability of proteins from rates of oxidation (SPROX), etc.
Leadart has established a complete chemical proteomics platform based on ABPP and AfBPP, providing target identification and validation services for active compounds, filling the gap in the field of domestic proteomics services. In addition, Leadart also undertakes regular proteomics services.
Peptidomics

Peptide group refers to all endogenous polypeptides in biological tissues or body fluids, including signal molecules (such as cytokines, growth factors, and hormones), antibacterial peptides, and immune peptides. These small peptides have been confirmed by more and more studies to have important biological significance. Peptidomics is a discipline that studies the structure, function, change rules and related relationships of peptide groups. Peptidomics is a supplement to proteomics. Peptomics can search for peptide markers with biological significance and screen potential therapeutic drugs with biological activity.

Peptide groups refers to all endogenous peptides in biological tissues or body fluids, including signal molecules (such as cytokines, growth factors, and hormones), antibacterial peptides, and immune peptides. More and more studies have confirmed that these peptides have important biological significance.
Conventional Proteomics

The concept of proteome was first proposed by Australian scholars in 1995. It is derived from the hybridization of the two words protein and genome, which refers to a specific time and space. Genome, all the proteins expressed by a cell tissue or an organism. The study of proteomics is called proteomics, which is mainly to study the composition and activity of proteins in cells at the overall level. The specific research content of proteomics includes: protein qualitative and quantitative analysis, protein post-translational modification research, protein structure research, protein function research, small molecule-protein and protein-protein interaction research, etc. Quantitative analysis is divided into non-labeled quantification (label free) and labeled quantification (SILAC, iTraq, TMT, etc.).

The specific research contents of proteomics include: protein qualitative and quantitative analysis, protein post-translational modifications, protein structure, protein function, small molecule-protein and protein-protein interaction, etc. Quantitative methods contain label free and label(SILAC, iTRAQ, TMT, etc.).

DRUG SCREENING LIBRARY ^{Target Probe+}

A Probe for Every Protein

Drug Screening Active Compound Library

Phenols Active Compounds Library^{Target Probe+}

Product Name	Cat. No.	Compounds	Size (10 mM in Pre-dissolved in DMSO )
Phenols Active Compounds Library ^{Target Probe+}	LA-001	Active Compounds 265 M Series 472 C Series 623	96-well plates 10 μL/well, 20 μL/well, or 100 μL/well

Phenolic compounds mainly refer to a class of hydroxyl (-OH) or methoxy (-OMe) directly combined with aromatic base groups, which have strong antioxidant properties, antibacterial and enzyme properties, anti-tumor and other functions Natural products.

Polyphenol Active Compounds Library^{Target Probe+}

Product Name	Cat. No.	Compounds	Size (10 mM in Pre-dissolved in DMSO )
Polyphenol Active Compounds Library ^{Target Probe+}	LA-002	Active Compounds 198 M Series 427 C Series 522	96-well plates 10 μL/well, 20 μL/well, or 100 μL/well

Polyphenol compounds mainly refer to a class of natural products composed of two or more hydroxyl groups (-OH) or methoxy groups (-OMe) directly combined with aromatic hydrocarbon groups. They are widely found in plants and have strong antioxidant properties. Antibacterial and enzyme-inhibiting properties, anti-tumor and other multifunctional natural products. The main structure includes flavonoids, quinones, alkaloids, phenylpropanoids and other polyphenols.

Flavonoid Active Compounds Library^{Target Probe+}

Product Name	Cat. No.	Compounds	Size (10 mM in Pre-dissolved in DMSO )
Flavonoid Active Compounds Library ^{Target Probe+}	LA-003	Active Compounds 52 M Series 163 C Series 165	96-well plates 10 μL/well, 20 μL/well, or 100 μL/well

Flavonoids are widely found in plants in nature and belong to the secondary metabolites of plants. Flavonoids are a type of yellow pigment derived from flavonoids (2-phenylchromone) as the core. Flavonoids are widely present in plants and are natural products with strong antioxidant properties, antibacterial and enzyme properties, anti-tumor and other multi-functional natural products.

Jujube Active Compounds Library^{Target Probe+}

Product Name	Cat. No.	Compounds	Size (10 mM in Pre-dissolved in DMSO )
Jujube Active Compounds Library ^{Target Probe+}	LA-004	Active Compounds 27 M Series 76 C Series 113	96-well plates 10 μL/well, 20 μL/well, or 100 μL/well

Zizyphus jujuba dates (zizyphus jujuba dates) are the fruits of the jujube tree (zizyphus iujuba Mill) of the genus Rhamnaceae. Modern pharmacology has found that red dates contain a variety of active natural products, such as carbohydrates, vitamins, triterpenoids, cyclic adenosine phosphate and polyphenols.

Salicylic Acid Active Compounds Library^{Target Probe+}

Product Name	Cat. No.	Compounds	Size (10 mM in Pre-dissolved in DMSO )
Salicylic Acid Active Compounds Library ^{Target Probe+}	LA-005	Active Compounds 17 M Series 25 C Series 48	96-well plates 10 μL/well, 20 μL/well, or 100 μL/well

Salicylates (Salicylates) drugs include aspirin and sodium salicylate (Sodium Salicylate), etc., due to its strong irritation, salicylic acid itself is only used as an antifungal and keratolytic agent.

Anti-Cancer Active Compounds Library^{Target Probe+}

Product Name	Cat. No.	Compounds	Size (10 mM in Pre-dissolved in DMSO )
Anti-Cancer Active Compounds Library ^{Target Probe+}	LA-006	Active Compounds 139 M Series 201 C Series 295	96-well plates 10 μL/well, 20 μL/well, or 100 μL/well

In currently, Cancer one of the most common and most serious diseases that directly endanger human life. According to incomplete statistics, nearly 20 million people in the world suffer from cancer every year, and about 10 million people die from cancer, accounting for the total number of deaths. About 1/4, this number is still growing rapidly.

Immunologically Active Compounds Library^{Target Probe+}

Product Name	Cat. No.	Compounds	Size (10 mM in Pre-dissolved in DMSO )
Immunologically Active Compounds Library ^{Target Probe+}	LA-008	Active Compounds 75 M series 131 C series 167	96-well plates 10 μL/well, 20 μL/well, or 100 μL/well

"Immunity" is an extremely important self-defense function of the human body. The human body can resist a huge variety of diseases by relying on its own immunity. Many active substances in natural products have immunological activity, such as polyphenols, flavonoids, and nitrogen-containing heterocyclic compounds.

Anti-Aging Active Compounds Library^{Target Probe+}

Product Name	Cat. No.	Compounds	Size (10 mM in Pre-dissolved in DMSO )
Anti-Aging Active Compounds Library ^{Target Probe+}	LA-009	Active Compounds 118 M Series 230 C Series 274	96-well plates 10 μL/well, 20 μL/well, or 100 μL/well

Case Study

Baicalin is an active ingredient in a class of natural products. It has been found to reduce the accumulation of lipids in cells. In order to study its corresponding protein targets and biological activity mechanisms, the researchers designed probe molecules corresponding to its active ingredients and carried out chemistry. The identification of proteomics found that the protein target corresponding to baicalin was CPT1A, and through subsequent binding experiments, in vitro activity experiments, and animal phenotyping experiments, the mechanism of its CPT1A target was verified, and it was successful. The anti-obesity and anti-steatosis effects of baicalin depend on its interaction with CPT1A.

AUTOMATION EQUIPMENT

Accelerate Drug Development

Scientific Research Automation Equipment and Development (ODM)

Keyone is a fully owned company of Leadart Technologies. Keyone is mainly engaged in the research and development, production, and sales of automated high-end equipment in the biomedical and biopharma field. Keyone has more than 38 patents and 28 software copyrights, and the commercially available products are listed here.Keyone is aspired to lead industry digitalization and automaton, free the hands of the researchers, providing automatic and intelligent solutions, and fill the gaps of automatic equipment needs. The company has customers from first-line research institutions, medical industry, health and beauty industries, scientific research institutions, medical institutions, pharmaceutical companies, biotechnology companies, third-party testing platforms and other customers.Free the hands of biologists and chemists.

NEWS

Company and Industry Updates

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