Case Study
Case Study

     

Baicalin is an active ingredient in a class of natural products. It has been found to reduce the accumulation of lipids in cells. In order to study its corresponding protein targets and biological activity mechanisms, the researchers designed probe molecules corresponding to its active ingredients and carried out chemistry. The identification of proteomics found that the protein target corresponding to baicalin was CPT1A, and through subsequent binding experiments, in vitro activity experiments, and animal phenotyping experiments, the mechanism of its CPT1A target was verified, and it was successful. The anti-obesity and anti-steatosis effects of baicalin depend on its interaction with CPT1A.


Step 1 Cellular phenotype: Baicalin reduces cell lipid accumulation

Step 2 Target identification: Quantitative chemical proteomics analysis confirms that CPT1A is the target of baicalin

Step 3 Combination verification: structural modeling and physiological verification of the CPT1A-baicalin interaction

Step 4 In vitro activity: Baicalin directly activates the enzyme activity of CPT1A

Step 5 Animal phenotype: Baicalin improves DIO and related metabolic disorders in mice

Step 6 Mechanism: Baicalin's anti-obesity and anti-steatosis effects depend on its interaction with CPT1A

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